3LHA
Crystal structure of mouse VPS26B(R240S/G241A/E242S) in spacegroup P41 21 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-27 |
Wavelength(s) | 0.95364 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 118.140, 118.140, 193.274 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.853 - 2.800 |
R-factor | 0.2539 |
Rwork | 0.252 |
R-free | 0.28380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r51 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.314 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.800 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.112 | 0.609 |
Number of reflections | 64306 | |
<I/σ(I)> | 19.7 | 3.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.6 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 2M sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |