3LH2
Crystal structure of HIV epitope-scaffold 4E10_1VI7A_S0_002_N 4E10 Fv complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 107 |
Detector technology | CCD |
Collection date | 2008-10-22 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.850, 145.950, 78.550 |
Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
Resolution | 26.700 - 2.650 |
R-factor | 0.22426 |
Rwork | 0.222 |
R-free | 0.27023 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Computationally-derived model of the epitope-scaffold Fv complex with the Fv based on PDB ID 1TZG. |
RMSD bond length | 0.006 |
RMSD bond angle | 0.927 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.010 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.095 | 0.376 |
Number of reflections | 49017 | |
<I/σ(I)> | 9.4 | 2.7 |
Completeness [%] | 98.9 | 98.6 |
Redundancy | 3.88 | 3.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Na acetate, imidazole, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |