3LGY
R178A mutant of the DegS-deltaPDZ protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-04-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5416 |
Spacegroup name | H 3 |
Unit cell lengths | 70.216, 70.216, 119.228 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.462 - 2.700 |
R-factor | 0.237 |
Rwork | 0.236 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.803 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.058 | 0.039 | 0.201 |
Number of reflections | 5938 | ||
<I/σ(I)> | 15.6 | ||
Completeness [%] | 98.2 | 99.5 | 87.5 |
Redundancy | 10.2 | 11.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 50 mM Sodium Cacodylate, 50-125 mM Sodium Citrate, 10-20 % isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |