3LGW
H198P/T167V double mutant of DegS-deltaPDZ protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-04-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5416 |
Spacegroup name | H 3 |
Unit cell lengths | 70.664, 70.664, 121.036 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.665 - 2.500 |
R-factor | 0.2133 |
Rwork | 0.212 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.844 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.034 | 0.029 | 0.138 |
Number of reflections | 7346 | ||
<I/σ(I)> | 25.9 | ||
Completeness [%] | 94.3 | 99.4 | 89.4 |
Redundancy | 4.2 | 4.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 50 mM Sodium Cacodylate, 50-125 mM Sodium Citrate, 10-20 % isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |