3LGT
Y162A/H198P double mutant of DegS-deltaPDZ protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5416 |
| Spacegroup name | H 3 |
| Unit cell lengths | 70.647, 70.647, 120.054 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.802 - 2.680 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.822 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.770 |
| High resolution limit [Å] | 2.670 | 5.750 | 2.670 |
| Rmerge | 0.035 | 0.023 | 0.212 |
| Number of reflections | 5910 | ||
| <I/σ(I)> | 20.9 | ||
| Completeness [%] | 93.7 | 97.2 | 80.9 |
| Redundancy | 4.5 | 4.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | 50 mM Sodium Cacodylate, 50-125 mM Sodium Citrate, 10-20% isopropanol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
