3LGM
Crystal structure of reduced IsdI in complex with heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.040, 68.030, 70.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.870 - 1.880 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zdp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.348 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 70.750 |
| High resolution limit [Å] | 1.880 |
| Number of reflections | 23396 |
| Completeness [%] | 99.8 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% polyethylene glycol 3350, 0.2M MgCl2, 0.1M Bis-Tris buffer pH 5.5, and soaked in 50mM sodium dithionite for 10 minutes with a cryoprotectant of 10% ethylene glycol before freezing, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
