3LGI
Structure of the protease domain of DegS (DegS-deltaPDZ) at 1.65 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.384, 72.762, 128.877 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.681 - 1.652 |
R-factor | 0.1534 |
Rwork | 0.152 |
R-free | 0.18150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.039 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.680 |
High resolution limit [Å] | 1.650 | 4.480 | 1.650 |
Rmerge | 0.069 | 0.041 | 0.481 |
Number of reflections | 79543 | ||
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.6 | 99.4 | 94.8 |
Redundancy | 12.9 | 13.4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 40-100 mM Sodium Cacodylate, 10-20 % isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |