3LGE
Crystal structure of rabbit muscle aldolase-SNX9 LC4 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-11 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.028, 118.175, 175.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.880 - 2.200 |
R-factor | 0.153 |
Rwork | 0.151 |
R-free | 0.18900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ot0 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Rmerge | 0.143 | 0.063 | 0.526 |
Number of reflections | 91509 | ||
<I/σ(I)> | 4.5 | ||
Completeness [%] | 98.7 | 99.8 | 89.8 |
Redundancy | 5.9 | 6.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG-MME 550, MgCl2, pH 7, Vapor Diffusion, Hanging drop, temperature 277K |