3LGC
Crystal Structure of Glutaredoxin 1 from Francisella tularensis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 61 |
| Unit cell lengths | 66.437, 66.437, 63.116 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.200 - 2.770 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.462 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.4_147)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.200 | 2.820 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Number of reflections | 4103 | |
| <I/σ(I)> | 7.2 | 6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M magnesium chloride, 0.1 M Tris pH 8.5, 20 % PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
