3LGB
Crystal Structure of the Fe-S Domain of the yeast DNA primase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Collection date | 2009-06-16 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 |
| Unit cell lengths | 86.570, 86.570, 141.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.340 - 1.540 |
| R-factor | 0.1547 |
| Rwork | 0.154 |
| R-free | 0.16430 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.340 | 1.620 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.101 | 0.778 |
| Number of reflections | 88345 | |
| <I/σ(I)> | 21.6 | 4.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 22.4 | 20.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M TrisHCl pH 7.5, 1mM Zn acetate, 11% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
