3LFZ
Crystal Structure of Protein MJ1225 from Methanocaldococcus jannaschii, a putative archaeal homolog of g-AMPK.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9395 |
Spacegroup name | H 3 2 |
Unit cell lengths | 109.144, 109.144, 142.414 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 78.810 - 2.200 |
R-factor | 0.25324 |
Rwork | 0.251 |
R-free | 0.28525 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kpd |
RMSD bond length | 0.007 |
RMSD bond angle | 1.230 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 78.810 |
High resolution limit [Å] | 2.200 |
Number of reflections | 15961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ligand soaks, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |