3LFF
Human p38 MAP Kinase in Complex with RL166
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2009-12-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.978946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.390, 68.750, 74.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.500 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zyj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.243 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.045 | 0.351 |
Number of reflections | 52551 | 9127 |
<I/σ(I)> | 20.26 | 4.6 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.65 | 4.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |