3LF9
Crystal structure of HIV epitope-scaffold 4E10_D0_1IS1A_001_C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 107 |
| Detector technology | CCD |
| Collection date | 2008-06-04 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.660, 75.360, 79.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.370 - 2.000 |
| R-factor | 0.20697 |
| Rwork | 0.204 |
| R-free | 0.25969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Computationally-derived model of the epitope-scaffold based on 1IS1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.004 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.640 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.185 |
| Number of reflections | 8462 | |
| <I/σ(I)> | 18.6 | 4 |
| Completeness [%] | 98.3 | 89.9 |
| Redundancy | 4.53 | 2.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 4000, Ca chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
