3LEQ
The Crystal Structure of the Roadblock/LC7 domain from Streptomyces avermitillis to 1.85A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.466, 29.824, 40.752 |
| Unit cell angles | 90.00, 104.89, 90.00 |
Refinement procedure
| Resolution | 43.230 - 1.850 |
| R-factor | 0.21693 |
| Rwork | 0.216 |
| R-free | 0.23588 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.238 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (refmac_5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.083 | 0.063 | 0.561 |
| Number of reflections | 8867 | ||
| <I/σ(I)> | 16 | ||
| Completeness [%] | 98.5 | 91.5 | 99 |
| Redundancy | 3.6 | 3.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 289 | 2M Ammonium phosphate, 0.1M Tris pH 8.5, vapor diffusion, temperature 289K |
