3LEC
The Crystal Structure of a protein in the NADB-Rossmann Superfamily from Streptococcus agalactiae to 1.8A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.444, 62.871, 61.784 |
| Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
| Resolution | 37.930 - 1.800 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.24800 |
| Starting model (for MR) | 3gnl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.306 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.084 | 0.551 |
| Number of reflections | 19085 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 289 | 2M Ammonium sulfate, 0.1M Tris pH 7.0, 0.2M Lithium sulfate, vapor diffusion, temperature 289K |
