3LE5
Crystal structure of HPr dimer from Thermoanaerobacter tengcongensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-07 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9798 |
| Spacegroup name | I 41 |
| Unit cell lengths | 76.090, 76.090, 92.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.045 - 2.000 |
| R-factor | 0.2171 |
| Rwork | 0.215 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mu4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17747 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.6 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.05M MgCl2, 0.1M HEPES pH7.5, 30% PEG MME550, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
