3LE4

Crystal structure of the DGCR8 dimerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	39.952, 39.952, 80.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.250 - 1.701
R-factor	0.1823
Rwork	0.181
R-free	0.21300
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.327
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.300	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	7721
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.2 M ammonium acetate, 100 mM Bis-Tris pH 5.5, 25% polyethylene glycol 3350, 10 mM nicotinamide adenine dinucleotide, VAPOR DIFFUSION, HANGING DROP, temperature 291K