3LE1
Crystal structure of apoHPr monomer from Thermoanaerobacter tengcongensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9002 |
| Spacegroup name | P 42 |
| Unit cell lengths | 57.860, 57.860, 42.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.042 - 1.513 |
| R-factor | 0.2198 |
| Rwork | 0.218 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 21853 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% PEG 400, 0.1M Na Acetate pH4.6, 0.1M Cadmium Chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
