3LDZ
Crystal structure of human STAM1 VHS domain in complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Detector technology | CCD |
Collection date | 2009-02-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.610, 112.130, 68.660 |
Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
Resolution | 37.960 - 2.600 |
R-factor | 0.226 |
Rwork | 0.225 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.732 | 37.900 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 30561 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 95.5 | 93.4 |
Redundancy | 3.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2M Na thiocyanate, 20% PEG3350, 0.1M imidazole pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |