3LDZ
Crystal structure of human STAM1 VHS domain in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Collection date | 2009-02-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.610, 112.130, 68.660 |
| Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
| Resolution | 37.960 - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.732 | 37.900 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 30561 | |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 95.5 | 93.4 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2M Na thiocyanate, 20% PEG3350, 0.1M imidazole pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
