3LDS

Crystal structure of RB69 gp43 with DNA and dATP opposite 8-oxoG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-01
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	81.011, 121.002, 128.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.820 - 3.000
R-factor	0.24407
Rwork	0.242
R-free	0.29496
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ig9
RMSD bond length	0.007
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0041)

Data quality characteristics

	Overall
High resolution limit [Å]	3.000
Number of reflections	26810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	297	3% PEG 20000, 100mM sodium acetate, 100mM manganese acetate, 100mM Tris-HCl, 1% glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K