3LDK

Crystal Structure of A. japonicus CB05

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2008-12-30
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	99.350, 110.967, 66.129
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.229
Rwork	0.224
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200	2.200
Rmerge	0.053
Number of reflections	36134
<I/σ(I)>	30.3
Completeness [%]	95.7	98.1
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	8% PEG, 0.1M Sodium acetate, 150mM LiCl, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	8% PEG, 0.1M Sodium acetate, 150mM LiCl, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K