3LDA
Yeast Rad51 H352Y Filament Interface Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320357 |
Spacegroup name | P 61 |
Unit cell lengths | 78.905, 78.905, 130.333 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
Rwork | 0.200 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1szp |
RMSD bond length | 0.006 |
RMSD bond angle | 1.347 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | CNS (1.21) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.130 | 0.663 |
Number of reflections | 14791 | |
<I/σ(I)> | 18.03 | 2.03 |
Completeness [%] | 92.8 | 98.2 |
Redundancy | 9.6 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 35% (v/v) Pentaerythritol propoxylate (5/4 PO/OH), 300mM KCl, 50mM HEPES, 20mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |