3LD4
Urate oxidase complexed with 8-nitro xanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.964 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 78.582, 95.045, 104.371 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.350 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.22500 |
Structure solution method | AB INITIO |
Starting model (for MR) | 3l8w |
RMSD bond length | 0.027 |
RMSD bond angle | 0.072 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.046 | 0.164 |
Number of reflections | 81256 | |
<I/σ(I)> | 25 | 4.4 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 7.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 8 | 291 | 0.05M TRIS BUFFER PH 8, 8% PEG 8000, 0.02M NACL , BATCH, temperature 291K |