3LD4
Urate oxidase complexed with 8-nitro xanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.964 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.582, 95.045, 104.371 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.350 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.22500 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 3l8w |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.072 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.046 | 0.164 |
| Number of reflections | 81256 | |
| <I/σ(I)> | 25 | 4.4 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 7.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 8 | 291 | 0.05M TRIS BUFFER PH 8, 8% PEG 8000, 0.02M NACL , BATCH, temperature 291K |
