3LD1
Crystal Structure of IBV Nsp2a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Detector technology | CCD |
| Collection date | 2008-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 64 |
| Unit cell lengths | 114.179, 114.179, 60.979 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.140 - 2.498 |
| R-factor | 0.219 |
| Rwork | 0.216 |
| R-free | 0.28400 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.075 | 0.390 |
| Number of reflections | 17086 | |
| <I/σ(I)> | 30.1 | 4 |
| Completeness [%] | 95.0 | 83.2 |
| Redundancy | 13.8 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 12% (w/v) PEG8000, 10% (v/v) Ethylene Glycol, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, temperature 291K |
