3LD1
Crystal Structure of IBV Nsp2a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Detector technology | CCD |
Collection date | 2008-10-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 64 |
Unit cell lengths | 114.179, 114.179, 60.979 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.140 - 2.498 |
R-factor | 0.219 |
Rwork | 0.216 |
R-free | 0.28400 |
Structure solution method | SAD |
RMSD bond length | 0.026 |
RMSD bond angle | 2.217 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.075 | 0.390 |
Number of reflections | 17086 | |
<I/σ(I)> | 30.1 | 4 |
Completeness [%] | 95.0 | 83.2 |
Redundancy | 13.8 | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 12% (w/v) PEG8000, 10% (v/v) Ethylene Glycol, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, temperature 291K |