3LCP
Crystal structure of the carbohydrate recognition domain of LMAN1 in complex with MCFD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 |
| Unit cell lengths | 58.602, 58.602, 396.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.450 |
| R-factor | 0.19878 |
| Rwork | 0.196 |
| R-free | 0.24726 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r1z |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.472 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.113 | 0.280 |
| Number of reflections | 28026 | |
| <I/σ(I)> | 17.6 | 5.8 |
| Completeness [%] | 98.9 | |
| Redundancy | 11.8 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 205 PEG6000, 0.1 M Ammonium Chloride 0.1 M Hepes, pH 7., VAPOR DIFFUSION, SITTING DROP, temperature 277K |
