3LCK
THE KINASE DOMAIN OF HUMAN LYMPHOCYTE KINASE (LCK), ACTIVATED FORM (AUTO-PHOSPHORYLATED ON TYR394)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-21 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.040, 73.620, 91.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19900 |
| Structure solution method | MIR, MOLECULAR REPLACEMENT, MAD |
| Starting model (for MR) | 1irk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.860 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.028 * | 0.047 * |
| Number of reflections | 31599 | |
| <I/σ(I)> | 37 | 21.8 |
| Completeness [%] | 99.0 | 96.1 |
| Redundancy | 8.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.51 (mg/ml) | |
| 2 | 1 | drop | ATP | 0.080 (mM) | |
| 3 | 1 | drop | substrate peptide | 0.080 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 0.6 (M) | |
| 5 | 1 | drop | Bis-Tris | 0.05 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 7 | 1 | reservoir | bis-Tris | 0.1 (M) |
