3LCA
Structure of Tom71 complexed with Hsp70 Ssa1 C terminal tail indicating conformational plasticity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-10-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.148, 97.971, 129.102 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.190 |
R-factor | 0.22942 |
Rwork | 0.227 |
R-free | 0.27112 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fp4 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.903 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.270 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.095 | 0.430 |
Number of reflections | 33761 | |
<I/σ(I)> | 30.8 | 2.5 |
Completeness [%] | 98.6 | 89.5 |
Redundancy | 5.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% Ethylene Glycol, 20% PEG 6000, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |