3LC9
Ricin A-chain variant 1-33/44-198 with engineered disulfide bond
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-21 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.493, 72.307, 94.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.360 - 2.280 |
| R-factor | 0.21511 |
| Rwork | 0.214 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3BJG |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.121 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.360 | 2.380 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 8333 | |
| <I/σ(I)> | 19.05 | 5.52 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.01 | 11.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 290 | Protein buffer: 50mM MES pH 6.4, 200mM NaCl Crystallization Solution: 0.17M Ammonium Sulfate, 25.5% w/v PEG 4000, 15% v/v Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
