3LC6
The alternative conformation structure of isocitrate dehydrogenase kinase/phosphatase from E. Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.145, 133.757, 187.339 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.100 |
| R-factor | 0.289 |
| Rwork | 0.287 |
| R-free | 0.33200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.582 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.101 | 0.280 |
| Number of reflections | 27965 | |
| <I/σ(I)> | 15.1 | 4.8 |
| Completeness [%] | 98.5 | |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 295 | 12% PEG 8000, 0.001M calcium chloride, 0.2M magnesium chloride, 0.1M MES 15% Glycerol, 0.002M DTT, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
