Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.030, 118.840, 65.980 |
| Unit cell angles | 90.00, 132.57, 90.00 |
Refinement procedure
| Resolution | 34.108 - 1.800 |
| Rwork | 0.193 |
| R-free | 0.21120 |
| Structure solution method | previous isomorphous structure |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.264 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.3.9) |
| Refinement software | REFMAC (refmac_5.5.0096) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.000 | 48.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
| Rmerge | 0.042 | 0.020 | 0.640 |
| Total number of observations | 5410 | 25685 | |
| Number of reflections | 47130 | ||
| <I/σ(I)> | 14.0065 | ||
| Completeness [%] | 99.8 | 96.36 | 99.85 |
| Redundancy | 3.78 | 3.68 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 0.2 M Lithium Sulfate, 26% PEG 3350, 0.1 M Bis/Tris pH 5.5, 1mM TCEP, 10 mM L-Histidine, vapor diffusion, temperature 293K |
