3LBG
Urate oxidase complexed with 8-thio xanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.948 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.637, 95.471, 104.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.500 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.24100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3l8w |
RMSD bond length | 0.035 |
RMSD bond angle | 0.080 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.800 | 1.650 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.041 | 0.880 |
Number of reflections | 60541 | |
<I/σ(I)> | 29.4 | 5.1 |
Completeness [%] | 94.5 | 96 |
Redundancy | 4.2 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 291 | 0.05M TRIS BUFFER, PH 8, 10 % PEG 8000, temperature 291K |