3LAG
The crystal structure of a functionally unknown protein RPA4178 from Rhodopseudomonas palustris CGA009
Replaces: 2OZIExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.871, 59.243, 65.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.981 - 1.150 |
| R-factor | 0.1871 |
| Rwork | 0.185 |
| R-free | 0.22050 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.188 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 1.160 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.135 | 0.492 |
| Number of reflections | 59329 | |
| <I/σ(I)> | 125.76 | 2.36 |
| Completeness [%] | 99.1 | 90 |
| Redundancy | 8.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M CaCl2, 0.1M HEPES, 15% PEG400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
