3LA7
Crystal structure of NtcA in apo-form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-08 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9791, 0.9792, 0.9795, 0.9700 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.506, 85.624, 171.959 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.21712 |
| Rwork | 0.215 |
| R-free | 0.26397 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.410 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.534 |
| Number of reflections | 39299 | |
| <I/σ(I)> | 18.2 | 4.5 |
| Completeness [%] | 99.0 | 98.2 |
| Redundancy | 10.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 289 | 30-40%(v/v) PEG 300, 0.1M NaAc pH4.5, 0.2M NaCl, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
