3LA2
Crystal structure of NtcA in complex with 2-oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.140, 70.260, 149.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.730 - 2.600 |
| R-factor | 0.22424 |
| Rwork | 0.222 |
| R-free | 0.26848 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3la3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.386 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.990 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | 0.697 |
| Number of reflections | 22720 | |
| <I/σ(I)> | 13.3 | 2.6 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 289 | 0.05M (NH4)2SO4, 25% pentaerythritol ethoxylate (3/4, EO/OH), pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
