3LA0
Crystal Structure of UreE from Helicobacter pylori (metal of unknown identity bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-19 |
| Detector | MAR165 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.557, 85.557, 202.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.060 - 2.860 |
| R-factor | 0.27957 |
| Rwork | 0.278 |
| R-free | 0.30486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UreE in apo form (PDB code 3L9Z) |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.844 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.060 | 2.960 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmerge | 0.120 | 0.347 |
| Number of reflections | 18190 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.4 | 97.3 |
| Redundancy | 23.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 295 | 16% PEG 8000, 5% glycerol, 0.1M Sodium Citrate pH 5.0, vapor diffusion, sitting drop, temperature 295K |
