3L9Z
Crystal Structure of UreE from Helicobacter pylori (apo form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 131.011, 131.011, 51.267 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.880 - 2.080 |
R-factor | 0.22277 |
Rwork | 0.221 |
R-free | 0.25488 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.309 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.880 | 50.000 | 2.150 |
High resolution limit [Å] | 2.080 | 4.480 | 2.080 |
Rmerge | 0.050 | 0.025 | 0.496 |
Number of reflections | 16040 | ||
<I/σ(I)> | 18.7 | ||
Completeness [%] | 99.8 | 98.5 | 99.5 |
Redundancy | 15.7 | 19.1 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 17% PEG 8000, 0.1M Sodium Citrate pH 5.0, vapor diffusion, sitting drop, temperature 295K |