3L9Y
Crystal structures of holo and Cu-deficient Cu/ZnSOD from the silkworm Bombyx mori and the implications in Amyotrophic lateral sclerosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-10 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 64 |
| Unit cell lengths | 111.493, 111.493, 39.886 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.22491 |
| Rwork | 0.223 |
| R-free | 0.25759 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1azv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.004 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.297 |
| Number of reflections | 26262 | |
| <I/σ(I)> | 21.3 | 5.2 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 2.0M (NH4)2SO4, 0.1M Tris-HCl, pH 8.5, 10% glycerol, 1mM CuCl2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
