3L9X
KefC C-terminal domain in complex with KefF and ESG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-04 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.949, 85.941, 189.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.260 - 2.100 |
R-factor | 0.20445 |
Rwork | 0.203 |
R-free | 0.23567 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eyw |
RMSD bond length | 0.016 |
RMSD bond angle | 1.449 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.091 | 0.440 |
Number of reflections | 62001 | |
<I/σ(I)> | 7.8 | |
Completeness [%] | 98.5 | 91 |
Redundancy | 4.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 298 | 9% PEG 3350, 100mM ammonium sulfate, 100mM Bis-Tris buffer, 50mM magnesium formate, 1mM NAD+, pH 5.5, temperature 298K, VAPOR DIFFUSION, HANGING DROP |