3L9V
Crystal Structure of Salmonella enterica serovar Typhimurium SrgA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.956661 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.445, 80.441, 104.953 |
| Unit cell angles | 90.00, 124.42, 90.00 |
Refinement procedure
| Resolution | 36.477 - 2.151 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.069 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (AutoSol) |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 67898 | |
| <I/σ(I)> | 15.3 | 5.63 |
| Completeness [%] | 98.1 | 97.2 |
| Redundancy | 15.5 | 15.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.45M sodium malonate, 0.5% (w/v) Jeffamine ED-2001, 100mM HEPES pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
