3L9P
Crystal Structure of the Anaplastic Lymphoma Kinase Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.058, 56.904, 103.607 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.780 - 1.800 |
| R-factor | 0.19165 |
| Rwork | 0.190 |
| R-free | 0.22636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p4o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.263 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.065 |
| Number of reflections | 27817 | |
| <I/σ(I)> | 22.17 | |
| Completeness [%] | 95.5 | 62.8 |
| Redundancy | 3.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 20% PEG 8000, 0.1M HEPEs pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
