3L9H
X-ray structure of mitotic kinesin-5 (KSP, KIF11, Eg5)in complex with the hexahydro-2H-pyrano[3,2-c]quinoline EMD 534085
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9001 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.950, 79.718, 69.513 |
| Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.25770 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.237 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 51847 | |
| Completeness [%] | 88.3 | 43.5 |
| Redundancy | 2.5 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 17-18% w/v PEG3350, 0.23-0.26M ammonium citrate pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
