3L9A
Structure of the C-terminal domain from a Streptococcus mutans hypothetical
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915, 0.97942 |
Spacegroup name | I 4 |
Unit cell lengths | 64.080, 64.080, 39.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.290 - 1.300 |
R-factor | 0.13462 |
Rwork | 0.134 |
R-free | 0.15490 |
Structure solution method | SAD |
Starting model (for MR) | none |
RMSD bond length | 0.008 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.290 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.038 | 0.113 |
Number of reflections | 20493 | |
<I/σ(I)> | 59.333 | 10.5 |
Completeness [%] | 90.9 | 49.9 |
Redundancy | 6.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M Hepes ph7.5, 0.2M Ammonium Sulfate, 25% PEG3350, 1/10 Papain. Cryoprotected with crystallization solution plus 8% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |