3L8U
Crystal structure of SMU.1707c, a putative rRNA methyltransferase from streptococcus mutans UA159
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-22 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 |
Unit cell lengths | 82.277, 82.277, 70.992 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.600 - 2.000 |
R-factor | 0.20763 |
Rwork | 0.206 |
R-free | 0.23896 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3e5y |
RMSD bond length | 0.020 |
RMSD bond angle | 1.633 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 31772 |
Completeness [%] | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.5M (NH4)2SO4, 0.1M Bis-tris pH7.0, 0.7% beta-OG , VAPOR DIFFUSION, SITTING DROP, temperature 293K |