3L8Q
Structure analysis of the type II cohesin dyad from the adaptor ScaA scaffoldin of Acetivibrio cellulolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.930 |
Spacegroup name | P 1 |
Unit cell lengths | 59.225, 73.912, 90.793 |
Unit cell angles | 100.25, 94.26, 112.15 |
Refinement procedure
Resolution | 29.630 - 1.570 |
R-factor | 0.1805 |
Rwork | 0.178 |
R-free | 0.22554 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zv9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.333 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.600 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.077 | 0.446 |
Total number of observations | 707451 | |
Number of reflections | 192139 | |
<I/σ(I)> | 23.9 | 2.1 |
Completeness [%] | 97.0 | 95.4 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.15M citric acid pH 3.5, 16% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |