3L8N
Crystal Structure of a domain of Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BrefeldinA-inhibited GEP 2) from Homo sapiens (Human). Northeast Structural Genomics Consortium target id HR5562A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 |
Unit cell lengths | 53.929, 53.929, 75.711 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.650 - 2.860 |
R-factor | 0.2362 |
Rwork | 0.236 |
R-free | 0.29010 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.539 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.910 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.073 | 0.343 |
Number of reflections | 13133 | |
Completeness [%] | 97.8 | 99.3 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | PEG1000, sodium citrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |