3L8D

Crystal structure of methyltransferase from Bacillus Thuringiensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	46.079, 99.281, 121.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.820 - 1.700
R-factor	0.211
Rwork	0.211
R-free	0.22500
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.700
Rmerge	0.076
Number of reflections	29613
Completeness [%]	94.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	10% PEG 8000, 0.1M SODIUM/POTASSIUM PHOSPHATE, 0.2M SODIUM CHLORIDE, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K