3L8A
Crystal structure of MetC from Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-24 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.377, 64.346, 99.315 |
| Unit cell angles | 90.00, 100.93, 90.00 |
Refinement procedure
| Resolution | 24.567 - 1.539 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.016 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.539 | 1.540 |
| Number of reflections | 106510 | |
| Completeness [%] | 97.9 | 87.1 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 25% PEG MME 550, 0.1M Na Citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
