3L89
Human Adenovirus type 21 knob in complex with domains SCR1 and SCR2 of CD46 (membrane cofactor protein, MCP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9395 |
| Spacegroup name | P 1 |
| Unit cell lengths | 94.530, 107.710, 154.100 |
| Unit cell angles | 90.01, 90.10, 104.70 |
Refinement procedure
| Resolution | 47.991 - 3.500 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ad21 fiber knob model PDB ENTRY 2O39: CD46 SCR1-SCR2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_288) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.179 | 0.355 |
| Number of reflections | 71365 | |
| <I/σ(I)> | 8.9 | 3.6 |
| Completeness [%] | 96.2 | 74.9 |
| Redundancy | 3.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 6% PEG 8000, 0.1M LiCl, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
