3L7O
Crystal structure of Ribose-5-phosphate isomerase A from streptococcus mutans UA159
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.969, 101.089, 98.267 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.800 - 1.700 |
| R-factor | 0.19812 |
| Rwork | 0.197 |
| R-free | 0.21355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uj4 |
| RMSD bond length | 0.038 |
| RMSD bond angle | 2.536 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 106808 | |
| Completeness [%] | 98.0 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 10% PEG4000, 0.1M NaAC, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
