3L7I
Structure of the Wall Teichoic Acid Polymerase TagF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97934 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 223.739, 223.739, 100.691 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.840 - 2.700 |
R-factor | 0.2128 |
Rwork | 0.210 |
R-free | 0.26030 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.277 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.840 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.099 | 0.584 |
Number of reflections | 419093 | |
<I/σ(I)> | 13.3 | 1.5 |
Completeness [%] | 98.1 | 93.3 |
Redundancy | 6.1 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |